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Avidity生物素蛋白連接酶-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-11      瀏覽次數(shù):162

Avidity生物素蛋白連接酶

Avidity LLC 成立于 1996 年,位于色彩繽紛的科羅拉多州。

生wu素化菌株;AviTag 載體;生wu素化重組蛋白我們AviTag™技術(shù)是利用大腸桿菌的生wu素連接酶(BIRA),在一個(gè)du特的15個(gè)氨基酸肽標(biāo)記上,對(duì)單一生wu素進(jìn)行高度靶向的酶結(jié)合。AviTag™序列為GLNDIFEAKIEWHE。定向在鏈霉親和素涂層表面,這創(chuàng)造了一個(gè)理想的表現(xiàn)分子結(jié)合相互作用。提供全套的蛋白、肽生wu素化的產(chǎn)品,其主要產(chǎn)品包括:生wu素蛋白連接酶(BirA);生wu素化菌株;AviTag 載體;生wu素化重組蛋白。


以下是Avidity公司的部分熱銷(xiāo)產(chǎn)品

產(chǎn)品名稱

貨號(hào)

規(guī)格

BirA Biotin-Protein Ligase

BIRA500

40ug: 2 vials of 20ug each

Bulk BirA Biotin-Protein Ligase

Bulk BIRA

300ug: 10 vials of 30ug each

Biotin Solution

BIO200

1.5 mL of 500uM d-biotin

Biotin Solution

BIO500

10 mL of 5 mM d-biotin

Positive Control Protein Substrate Kit

BIS300

1 Kit

BIS-300 positive and negative control protein kit

BIS-300

1 kit

Biotin AviTag™ Vectors (N-terminal)

pAN4

10 mg of plasmid DNA

Chemically Competent E. coli B Strain AVB101

CVB101

10 vials, 100 mL per vial

Efficient Biotinylation E. coli K12 Strain AVB100

AVB100

1 vial, containing 0.5 mL of glycerol stock

Anti-C-terminus AviTag™-conjugated Agarose Resin

AbCA

2.0 ml

Efficient Biotinylation E. coli B Strain AVB101

AVB101

1 vial, containing 0.5 mL of glycerol stock

Electrocompetent E. coli B Strain AVB101

EVB101

10 vials, 50 mL per vial

Electrocompetent E. coli K12 Strain AVB100

EVB100

10 vials, 50 mL per vial

Positive Control Plasmid

BIP300

2ug

Anti-C-terminus AviTag™ Antibody

AbC

100ul, 1 mg/ml

Biotinylated Gaussia princeps Luciferase

Gluc-10

10 ug

Biotinylated Gaussia princeps Luciferase

Gluc-100

100 ug

Streptavidin-conjugated Gaussia princeps Luciferase

SA-Gluc

50 ug

Native Coelenterazine Free Base

CTZ-1

1 mg

Biotinylated Renilla reniformis Green Fluorescent Protein

rrGFP-100

100 ug

Biotinylated Fluorescent Proteins(Twinkle-Avitag)

FP-495

100 ug

Biotinylated Fluorescent Proteins(Holly-Avitag)

FP-515

100 ug

Biotinylated Fluorescent Proteins(Dasher-Avitag )

FP-525

100 ug

Fluorescent Protein for detection of biotinylated molecules(SA-Twinkle)

SA-FP-495

100 ug

Fluorescent Protein for detection of biotinylated molecules(SA-Holly)

SA-FP-515

100 ug

Fluorescent Protein for detection of biotinylated molecules(SA-Cratchit)

SA-FP-545

100 ug

Fluorescent Protein for detection of biotinylated molecules(SA-Yukon)

SA-FP-563

100 ug

Fluorescent Protein for detection of biotinylated molecules(SA-Rudolph)

SA-FP-570

100 ug

Biotin AviTag™ Vectors (N-terminal)

pAN4, 5, or 6

10ug of plasmid DNA

Biotin AviTag™ Vectors (N-terminal)

pANs

one kit containing 10ug of each plasmid

Biotin AviTag™ Vectors (C-terminal)

pAC4, 5, or 6

10ug of plasmid DNA

Biotin AviTag™ Vectors (C-terminal)

pACs

one kit containing 10ug of each plasmid

Forward-sequence primer for Biotin AviTag™ Vectors

FASP

200 pmol

Reverse-sequence primer for Biotin AviTag™ Vectors

RASP

200 pmol

 

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